NMN induces lipolysis
The study found that NMN treatment reduced lipid accumulation in these cells. By examining the glycerol concentration in the cell culture medium, we found that NMN treatment enhanced lipolysis in adipocytes.
To determine the effect of NMN on lipid storage in mature white adipocytes, the researchers treated differentiated 3T3-L1 adipocytes with NMN and performed Oil red O staining. It was found that treatment with 0.5 mM NMN for four days significantly reduced lipid accumulation in differentiated 3T3-L1 adipocytes (Fig. 1A), although this concentration of NMN did not affect the viability of the cells (Fig. 1B). NMN treatment increased The concentration of glycerol in the medium of 3T3-L1 adipocytes was increased (Fig. 1C). These results suggest that NMN reduces lipid accumulation in 3T3-L1 adipocytes by enhancing lipolysis.
NMN inhibits adipocyte hypertrophy in subcutaneous adipose tissue of obese mice
NMN treatment significantly reduced the amount of subcutaneous fat in mice induced by a high-fat diet and decreased the size of adipocytes in subcutaneous fat. Consistent with changes in subcutaneous fat mass and adipocyte size, NMN treatment slightly but significantly increased ATGL expression in subcutaneous fat.
These results suggest that NMN may suppress high-fat diet-induced subcutaneous fat mass in mice by upregulating ATGL expression. The effect of NMN on lipid storage in adipocytes was revealed, suggesting that it might enhance lipolysis in adipocytes by regulating ATGL expression in the SIRT1-AMPK signaling pathway. This provides a new mechanism for understanding how NMN improves obesity and related metabolic disorders.
